JULIUS MÜLLER



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I'm a Oxford based bioinformatics analyst with several years of experience. My main expertise is the analysis and integration of large scale data sets such as from Next Generation Sequencing and microarrays as well as designing and implementing new bioinformatics software, tools and data bases.

As a bioinformatics analyst, I can help with:
  • Experimental design and power analysis
  • Raw data analysis and quality assessment
  • Statistical and biological data interpretation
  • Design and implementation of tailor made software packages and databases
  • Literature search and annotation
I am always interested in collaborative work and I am looking forward to hearing from you.

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I would appreciate any comments, contributions, critics or suggestions.

Work Experience
2014-now Bioinformatician, The Jenner Institute, University of Oxford, UK
2012-2014 Bioinformatics Consultant, Zurich, Switzerland
2009-2012 Research Fellow in Bioinformatics, IMCB, Singapore
Education
2005-2009 PhD, ETH, Zurich, Institute of Cell Biology. Thesis: Transcriptional dynamics in immune cells at hypoxic conditions.
1999-2005 Diploma in Biology, Karls-Ruprecht University, Heidelberg, Major Biochemistry/Biophysics. Thesis: mRNA stability and degradation.
Computer Skills
OS Linux, Cluster handling (PBS server), Windows
Programming Daily use during work: R, Python, MySQL
  Frequent use: Linux Tools (awk, sed …) , C
  Past projects: Matlab, Java, Perl, C++, VBA-Excel
  Web Development: HTML+CSS, JavaScript
Publications (up to date list)
Salvany L, Muller J, Guccione E, and Rorth P.
The core and conserved role of MAL is homeostatic regulation of actin levels.
Genes & Development, May 15, 2014; 28 (10)
Loh SL, Teh C, Muller J, Guccione E, Hong W and Korzh V.
Zebrafish yap1 plays a role in differentiation of hair cells in posterior lateral line.
Science Reports, Mar 6, 2014; 4:4289
Jachowicz J, Santenard A, Bender A, Muller J and Torres-Padilla ME.
Heterochromatin establishment at pericentromeres depends on nuclear position.
Genes & Development, Nov 15, 2013; 27(22):2427-32
Wang X*, Zhao Z*, Muller J, Iyu A, Khng AJ, Guccione E, Ruan Y, and Ingham PW. * Equally contributing
Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish.
Biology Open, Nov 15, 2013; 2(11):1203-13
Burton A*, Muller J*, Padilla-Longoria P, Guccione E and Torres-Padilla ME. * Equally contributing
Single-Cell Profiling of Epigenetic Modifiers Identifies PRDM14 as an Inducer of Cell Fate in the Mammalian Embryo.
Cell Reports, Nov 14, 2013; 5(3):687-701 (Video Featured Article)
Filipponi D, Muller J, Emelyanov A and Bulavin DV.
Wip1 Controls Global Heterochromatin Silencing via ATM/BRCA1-Dependent DNA Methylation.
Cancer Cell, Oct 14, 2013; 24(4):528-41 (Featured Article)
Windak R, Muller J, Felley A, Akhmedov A, Wagner EF, Pedrazzini T, Sumara G, and Ricci R.
The AP-1 Transcription Factor c-Jun Prevents Stress-Imposed Maladaptive Remodeling of the Heart.
PLoS One, Sep 10, 2013; 8(9)
Bezzi M, Teo SX, Muller J, Mok WC, Sahu SK, Vardy LA, Bonday ZQ and Guccione E.
Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery.
Genes & Development, Sep 1, 2013; 27(17):1903-16
Bard-Chapeau EA, Gunaratne J, Kumar P, Chua B, Muller J, Bard FA, Blackstock W, Copeland NG, and Jenkins NA.
EVI1 oncoprotein interacts with a large and complex network of proteins and integrates signals through protein phosphorylation.
PNAS, Jul 30, 2013; 110(31):2885-94
Kumpf S, Mihlan M, Goginashvili A, Grandl G, Gehart H, Godel A, Schmidt J, Muller J, Bezzi M, Ittner A, Guccione E, Wolfrum C, Ricci R.
Hairless promotes PPARγ expression and is required for white adipogenesis
EMBO Reports, Nov 6, 2012; 13(11):1012-20
Bard-Chapeau EA, Jeyakani J, Kok CH, Muller J, Chua B, Gunaratne J, Batagov A, Jenjaroenpun P, Kuznetsov VA, Wei CL, D'Andrea R, Bourque G, Jenkins NA and Copeland NG.
Ecotopic viral integration site 1 (EVI1) regulates multiple cellular processes important for cancer and is a synergistic partner for FOS protein in invasive tumors
PNAS, Feb 7, 2012; 109(6):2168-73
Migliori V, Muller J, Low D, Phalke S, Bezzi M, Gunaratne J, Mok WC, Capasso P, Bassi C, Cecatiello V, Kuznetsov V, Blackstock W, Amati B, Mapelli M and Guccione E.
Symmetric dimethylation of H3R2 is a newly identified histone mark that supports euchromatin maintenance
Nature Structural & Molecular Biology, Jan 8, 2012; 19(2):136-44 (Cover Article)
Järve A, Muller J, Kim IH, Rohr K, MacLean C, Fricker G, Massing U, Eberle F, Dalpke A, Fischer R, Trendelenburg MF and Helm M.
Surveillance of siRNA integrity by FRET imaging
Nucleic Acids Research, Sep 22, 2007; 35(18)
Kurschat WC, Muller J, Wombacher R and Helm M.
Optimizing splinted ligation of highly structured small RNAs
RNA, Oct 26, 2005; 11(12):1909-14

Experience

Task Experience Estimated Duration
Experimental planning; Sample size and power analysis good Hours
Sequencing; Mapping, standard QA and basic analysis extensive Hours
Microarray and QPCR; Standard QA and basic analysis extensive Hours
Biological interpretation and thorough data mining extensive Days-Weeks
Bioinformatics Software development extensive Days-Weeks
Annotation extensive Hours
Proteomics; SILAC good
Genomics: SNP finding, de novo assembly, GWAS good

Tools

Tool Sample
This section contains Bioinformatics related tools and code snippets which are freely available. This section will be expanded over time and tools will be made available on public repositories such as GitHub. Comments, contributions and critics or collaborations are very welcome.

Python Logo

  • +BED to ELAND converter
    • Description: A lightweight tool to convert a bed file to the widely used eland format as output from the CASAVA pipeline
    • Requirements: Python 2.7
    • Source: bed2eland.py (GitHub)

R Logo

  • +Calculation of Shapiro scores
    • Description: Two functions to calculate the divergence of Donors and Acceptors from a reference. Based on the method published by Shapiro et al in 1987.
    • Requirements: R 2.10+
    • Source: calculate_shapiro.R (GitHub)

  • +TransView, bioconductor package
    • Description: TransView provides efficient tools to generate, access and display read densities of sequencing based data sets such as from RNA-Seq and ChIP-Seq.
    • Requirements: R 2.15+; Bioconductor 2.11+
    • Source: TransView (Bioconductor)

  • +PANTHER.db, bioconductor package
    • Description: A set of annotation maps describing the entire Gene Ontology assembled using data from PANTHER.
    • Requirements: R 2.7+; Bioconductor 2.13+
    • Source: PANTHER.db (Bioconductor)

Code Snippets

  • +Malformed phred scores in SAM and SAM to BED conversion
    • Description: A typical scenario making the conversion from the richer SAM format to the slim BED format necessary, are malicious phred scores in the SAM file body which make a SAM to BAM conversion by samtools impossible. bedtools applied to the output of the following awk snippet can circumvent this problem. The command is embedded in an optional bash script which works on all sam files found in a directory and returns a sorted and indexed bam file. Do note that all original quality scores are lost using this pipeline.
    • Requirements: awk/bash;samtools;bedtools
    • Source:
      #!/bin/bash
      for i in $(find . -name '*.sam' -type f)
      do
      	echo "Processing $i"
      	awk '{OFS="\t";print($3,$4+1,$4+length($5),0,0,$2)}' $i | bedtools sort | bedtools bedtobam -g genome_file.fai > $i.bam ; samtools index $i.bam
      done